The vanadium nitrogenase of Azotobacter chroococcum. Purification and properties of the VFe protein
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The vanadium nitrogenase of Azotobacter chroococcum. Purification and properties of the VFe protein.
1. Nitrogenase activity of a strain of Azotobacter chroococcum lacking the structural genes for conventional nitrogenase (nifHDK) was separated into two components: an Fe-containing protein and a vanadoprotein. 2. The larger protein was purified to homogeneity by the criterion of electrophoresis of 10% (w/v) acrylamide gels in the presence of SDS. Two types of subunit, of Mr 50,000 and 55,000, ...
متن کاملThe vanadium nitrogenase of Azotobacter chroococcum
1. Nitrogenase activity of a strain of Azotobacter chroococcum lacking the structural genes of Monitrogenase (nifHDK) was associated with a V+Fe-containing protein and an Fe-containing protein [Robson, Eady, Richardson, Miller, Hawkins & Postgate (1986) Nature (London) 322, 388-390; Eady, Robson, Richardson, Miller & Hawkins (1987) Biochem. J. 244, 197-207]. 2. The Fe protein was purifed to hom...
متن کاملVanadium K-edge X-ray-absorption spectroscopy of the functioning and thionine-oxidized forms of the VFe-protein of the vanadium nitrogenase from Azotobacter chroococcum.
Vanadium K-edge X-ray-absorption spectra were collected for samples of thionine-oxidized, super-reduced (during enzyme turnover) and dithionite-reduced VFe-protein of the vanadium nitrogenase of Azotobacter chroococcum (Acl*). Both the e.x.a.f.s and the x.a.n.e.s. (X-ray-absorption near-edge structure) are consistent with the vanadium being present as part of a VFeS cluster; the environment of ...
متن کاملUnique features of the nitrogenase VFe protein from Azotobacter vinelandii.
Nitrogenase is an essential metalloenzyme that catalyzes the biological conversion of dinitrogen (N(2)) to ammonia (NH(3)). The vanadium (V)-nitrogenase is very similar to the "conventional" molybdenum (Mo)-nitrogenase, yet it holds unique properties of its own that may provide useful insights into the general mechanism of nitrogenase catalysis. So far, characterization of the vanadium iron (VF...
متن کاملNitrogenase in Azotobacter chroococcum and Klebsiella pneumoniae.
Blumberg, W. E. & Peisach, J. (1974) Arch. Biochem. Biophys. 162,502-512 Cammack, R. (1973) Biochem. Biophys. Res. Commun. 54,548-554 Cammack, R., Rao, K. K. & Hall, D. 0. (1971) Biochem. Biophys. Res. Commun. 44,s-14 Coffman, R. E. & Stavens, B. W. (1970) Biochem. Biophys. Res. Commun. 41,163-169 Fee, J. A. & Palmer, G. (1971) Biochim. Biophys. Actu 249,175-195 Genonde, Ic, Schlaak, M. E., Bri...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1987
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2440197